covid19_progression
Human renin gene transgenic mice with integration of 10 copies of a 15 kbp human DNA fragment containing the renin gene (hRN 8-12 mice). The renin-angiotensin system (RAS) is one of the determinant factors in regulating blood pressure and sodium absorption. RAS has been shown to accelerate various diseases such as renal failure, atherosclerosis, cardiac hypertrophy, and pulmonary hypertension. The reaction between renin, produced in the kidney, and its substrate angiotensinogen, produced in the liver, is the initial and rate-limiting step in the enzymatic cascade that generates angiotensin I. Angiotensin-converting enzyme in the lung converts angiotensin I to the potent vasoconstrictor angiotensin II (Ang II). The depositor reported that overactivation of the RAS induces hypertension in the F1 generation obtained from breeding between a female carrying the human renin gene (hRN+/+) and a male possessing the human angiotensinogen gene (hAG+/+). Moreover, female hAG+/+ mice when bred with male hRN+/+ mice are hypertensive specifically in late pregnancy, suggesting possible implications for preeclampsia or pregnancy-induced hypertension, a life-threatening disorder for human mothers and fetuses. hRN+/+ and hANG+/+ mice provide the opportunity to understand the role of the RAS in hypertension and other common diseases. hRN8-12 mice (RBRC01122), hAG 2-5 mice (RBRC01123), hRN8-12 x hAG2-5)F1 mice (RBRC02432).
B（1〜3か月）
<A HREF="https://mus.brc.riken.jp/en/mouse_of_month/may_2012_mm" target="_blank">Mouse of the Month May 2012</A><br><A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/blood_pressure.pdf" target="_blank">Systolic blood pressure</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01122_GB.pdf">Genetic Background</a>
Developed by Dr. Akiyoshi Fukamizu, University of Tsukuba in 1991. C57BL/6 background.
hRN 8-12
Hematological Research
true
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochemical and Biophysical Research Communications, 165, 826-832 (1989).
C57BL/6-Tg(REN)8-12Afu/AfuRbrc
<a href='https://brc.riken.jp/mus/pcr01122'>Genotyping protocol -PCR-</a>
human renin1 genomic DNA (10 exons, 9 introns and native promoter)
B (1-3 months)
深水 昭吉
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://akif2.tara.tsukuba.ac.jp/Top_iweb/Welcome_en.html" target="_blank">Lab HP (Japanese)</A>
Cell Biology Research
国立大学法人筑波大学
Carrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.
Carrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.
hRN 8-12
hRN 8-12
RBRC01122
Univ. Tsukuba
筑波大学・深水昭吉（1991）。C57BL/6背景。
条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochemical and Biophysical Research Communications, 165, 826-832 (1989).
ヒトレニン遺伝子のトランスジェニックマウス (hRN8-12マウス) 。ヒトレニン遺伝子を含む15kbpのDNA断片を10コピー保持する。レニンはヒト血管を収縮させ高血圧を維持する因子であるレニンーアンジオテンシン系のコンポーネント。腎臓から分泌されるレニンがアンジオテンシノーゲンに働き、アンジオテンシンIが生産され、さらに肝臓や各組織に含まれる変換酵素の作用を受けて強力な血管収縮作用をもつアンジオテンシンIIに変換することが知られている。hRN8-12の雌マウスとヒトアンジオテンシノーゲン遺伝子のトランスジェニックマウス (hAG 2-5) の雄を親に持つダブルトランスジェニックマウス ((hRN8-12 x hAG2-5)F1マウス) は高血圧症を呈する (THM, Tsukuba Hypertensive Mice) 。一方、hAG 2-5の雌マウスにhRN8-12の雄マウスを交配させると、妊娠後期に顕著な血圧上昇認められる (PAH, Pregnancy-Associated Hypertensive) 。hRN8-12マウス (RBRC01122) 、hAG 2-5マウス (RBRC01123) 、hRN8-12 x hAG2-5)F1 (RBRC02432) 。
Akiyoshi FUKAMIZU